Monoclonal antibodies reactive with human breast cancer

US4612282A · kind A · utility

Assignee

• The United States of America as Represented by the Secretary of the Department of Health and Human Services · US

Inventors

• Jeffrey Schlom · Potomac, US
• David Colcher · Potomac, US
• Marianna Nuti · Bethesda, US
• Patricia H. Hand · Washington, US
• Faye Austin · Annandale, US

Key dates

• Filing date: Mar 1, 1985
• Grant date: Sep 16, 1986
• Expiry date: Mar 1, 2005

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2474/20
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Monoclonal antibodies demonstrating a reactivity with human breast cancer are produced. The hybridoma cultures secreting immunoglobins are produced by hydridoma technology. Splenic lymphocytes of mice, immunized with membrane-enriched fractions of metastatic human mammary carcinoma tissue are fused with the NS-1 non-immunoglobulin-secreting murine myeloma cell line. Screening of immunoglobulin reactivities and double cloning of cultures yielded 11 monoclonal antibodies that demonstrated activities with the surface of human mammary tumor cells and not with the surface of apparently normal human tissues. These monoclonal antibodies aid in the diagnosis, prognosis and treatment of human breast cancer.