Patent · US Expired

Porphyrin derivatives as fluorescent markers for immunoassays

US4614723A · kind A · utility

133Cited by
3References
24Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMay 23, 1984
Grant dateSep 30, 1986
Priority date
Expiry dateMay 23, 2004

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S436/80
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

There are described novel porphyrin derivatives of the general formula ##STR1## wherein either R.sup.1 signifies the group ##STR2## and R.sup.2 signifies the group ##STR3## or R.sup.1 signifies the group ##STR4## and R.sup.2 signifies the group ##STR5## whereby X.sup.- signifies a halogen ion, an arylsulphonate ion, an alkylsulphonate ion or an alkyl sulphate ion, A signifies (C.sub.1-8)-alkylene and R.sup.3 signifies (C.sub.1-4)-alkyl, PA0 and sulphonic acid salts of compounds of formula I in which R.sup.1 signifies the group ##STR6## as well as a process for their manufacture and intermediates used in this process. Because of their water-solubility the novel porphyrin derivatives are suitable as label molecules for highly sensitive fluorescence immuno-assays, especially for time-resolving fluorescence immuno-assays. The coupling of the novel porphyrin derivatives to immunologically-active materials is carried out in the customary manner, for example with a water-soluble carbodiimide derivative.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.