Purification and activity assurance of precipitated heterologous proteins
US4620948A · kind A · utility
85Cited by
3References
16Claims
0Family size
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Key dates
| Filing date | Jun 1, 1984 |
| Grant date | Nov 4, 1986 |
| Priority date | — |
| Expiry date | Jun 1, 2004 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y304/21073
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for isolating and purifying refractile proteins from host cell culture including treating the host cell culture with a buffered solution to solubilize host protein, leaving refractile protein insoluble, treating the insoluble fraction with a strongly denaturing solution containing a reducing agent to solubilize the refractile protein, and renaturing the refractile protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.