Method and device for quantitative end point determination in immunofluorescence using microfluorophotometry
US4622291A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 11, 1984 |
| Grant date | Nov 11, 1986 |
| Priority date | — |
| Expiry date | Jun 11, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/112499
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention discloses a device and a process for quantitation of end point in the formation of fluorescent reaction product in microfluorophotometry. The process comprises: PA1 (a) incorporating a protective agent in a suitable mounting medium in an amount sufficient to reduce fading of fluorescent reaction product less than 25% of initial fluorescent intensity; PA1 (b) calibrating photometer used in said microscopy with a stable emitter; and PA1 (c) recording the intensity of fluorescence of said fluorescent reaction product by means for measuring light intensity. The invention also includes a device for calibration of the photometer and a kit comprising separate containers for suitable mounting medium, buffer, suitable immunofluorescent reagents, fading retardant means, a photometer calibrating device and the like and optional instructions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.