Immunological detection of bacterial pathogens with antibody-containing magnetic gel
US4677055A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 7, 1983 |
| Grant date | Jun 30, 1987 |
| Priority date | — |
| Expiry date | Dec 7, 2003 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/808
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A process for the immunobacteriological detection of pathogenic germs possessing specific antigenic determinants, and a kit for carrying out the process, with the aid of a magnetic gel to which are coupled anti-specific antigenic determinant antibodies, characterized by the following steps: PA0 (a) a biological medium supposedly contaminated by a pathogenic germ possessing a specific antigenic determinant is brought into contact with particles of the magnetic gel, PA0 (b) the particles of magnetic gel are then removed by magnetic means and PA0 (c) are inoculated onto a nutrient agar medium, PA0 (d) the agar medium is incubated to develop culture colonies of the pathogenic germs; PA0 (e) areas containing serum containing antibodies specific to the pathogenic germ to be detected are formed in the agar, PA0 (f) the germs are lysed by means of lysing agents, PA0 (g) the lysate is incubated with the serum for a time enabling the serological antibody-antigen reaction to take place by immunodiffusion on the agar, and PA0 (h) the quantity of germs contained in the biological medium is read directly, by virtue of the antigen-antibody reaction on the agar.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.