Patent · US Expired

Periplasmic protein recovery

US4680262A · kind A · utility

9Cited by
2References
24Claims
0Family size

Assignee

Inventors

Key dates

Filing dateOct 5, 1984
Grant dateJul 14, 1987
Priority date
Expiry dateOct 5, 2004

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S930/30
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Periplasmic proteins are recovered from transformed gram negative bacteria by a process comprising freezing and thawing the cells. Advantages are obtained by culturing the cells in phosphate-limiting media and by killing the cells prior to separation of periplasmic proteins.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.