Hybrid proteins produced by an ultrahigh prokaryotic expression system
US4691009A · kind A · utility
10Cited by
1References
3Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 26, 1984 |
| Grant date | Sep 1, 1987 |
| Priority date | — |
| Expiry date | Dec 26, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/825
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Hybrid useful proteins are prepared by a novel biological system comprising a prokaryotic host transformed with novel hybrid plasmids' .beta.-glucuronidase (BG) gene DNA and the desired protein gene DNA. Specifically exemplified are plasmids which comprise BG gene DNA and protein A DNA. E. coli K-12 derivative hosts transformed with plasmid pBG3-2.DELTA.N express >60% of the desired fusion protein having protein A-like biological activity. Other useful proteins can be expressed via the elegant highly efficient expression system of the subject invention.
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