Intact gene and method of excising and cloning same
US4707445A · kind A · utility
7Cited by
3References
5Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 31, 1984 |
| Grant date | Nov 17, 1987 |
| Priority date | — |
| Expiry date | Jul 31, 2004 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/445
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses isolated functionally intact whole genes and method of obtaining the same. The method includes treating genomic DNA with mung bean nuclease and formamide under controlled conditions. The invention also discloses cloning of said intact whole genes and a library of such cloned genes or any recombinations thereof. The invention is useful in deriving gene products as m-RNA, S-RNA, t-RNA, polypeptides and the like.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.