Efficient prokaryotic expression system using portions of the E. coli .beta .
US4721671A · kind A · utility
3Cited by
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27Claims
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Key dates
| Filing date | Dec 26, 1984 |
| Grant date | Jan 26, 1988 |
| Priority date | — |
| Expiry date | Dec 26, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/849
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A novel highly effective prokaryotic expression system is exemplified specifically by being used to produce the useful enzyme .beta.-glucuronidase (BG). This system uses a hybrid plasmid comprising BG gene promotor DNA. The level of expression of BG by an E. coli K-12 derivative host is in the 50% of total cellular protein range. The invention expression system also can be used to express other useful proteins, as disclosed herein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.