Process for the detection and assay by erythroadsorption
US4729961A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 11, 1985 |
| Grant date | Mar 8, 1988 |
| Priority date | — |
| Expiry date | Dec 11, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/827
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A process for the detection and assay of a biological substance by erythroadsorption by immobilizing a substance having a binding affinity for the biological substance to be assayed; incubating the immobilized substance with a liquid medium containing the biological substance to be assayed, forming a fixed substance; incubating the fixed substance with a coupling product comprising a specific ligand and a second ligand which binds with erythrocytes; adding erythrocytes; and determining the amount of erythrocytes bound to the coupling product. The determination of the amount of bound erythrocytes can be made visually with the naked eye or determined by lysis of the erythrocytes bound to the coupling product and quantitatively measuring the released hemoglobin. The specific ligand can be an antibody or an antigen and the second ligand capable of coupling erythrocytes can, for example, be red blood cell antibodies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.