Peptide antibodies and their use in detecting oncogene products

US4762706A · kind A · utility

Assignees

• Cetus Corporation · US
• Cold Spring Harbor Laboratory · US

Inventors

• Francis P. McCormick · Albany, US
• Gail L. Wong · Oakland, US
• Robin Clark · Palo Alto, US
• Norman Arnheim · Piedmont, US
• Danute E. Nitecki · Berkeley, US
• James R. Feramisco · Cold Spring Harbor, US

Key dates

• Filing date: Oct 17, 1984
• Grant date: Aug 9, 1988
• Expiry date: Oct 17, 2004

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Antibodies exhibit specificity toward single amino acid differences between proteins. These antibodies may be produced by synthesizing a peptide of the appropriate amino acid sequence contained in the protein, immunizing a host with the peptide, and extracting sera from the host to obtain the antibodies. The antibodies and the desired protein are then immunoprecipitated under conditions of partial denaturation to expose the epitope of the protein. The antibodies may be used for diagnostic or therapeutic purposes.