Mixture of amino acid derivatives, process of producing the mixture and use of the mixture for quantitative determination of the amino acids
US4784962A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 25, 1987 |
| Grant date | Nov 15, 1988 |
| Priority date | — |
| Expiry date | Feb 25, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/173845
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A mixture of fluorescently-excitable amino acid derivatives suitable for use in quantitative amino acid analysis is provided in which primary amino acids are present as derivatives of a first reagent and secondary amino acids are present as derivatives of a second different reagent. The process for preparing the mixture comprises the steps of derivatization of the primary amino acids of an amino acid sample with ortho-phthalaldehyde (OPA) and of derivatization of the secondary amino acids of the sample with fluorenylmethylchloroformate (FMOC) in the presence of acetonitrile. The mixture can be obtained automatically and is highly suitable for fast analysis by reversed-phase liquid chromatography.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.