Chemical capping by phosphitylation during oligonucleotide synthesis
US4816571A · kind A · utility
67Cited by
1References
16Claims
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Key dates
| Filing date | Jun 4, 1987 |
| Grant date | Mar 28, 1989 |
| Priority date | — |
| Expiry date | Jun 4, 2007 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07F9/142
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A method is provided for capping failure sequences in oligonucleotide synthesis by phosphitylation. A phosphite monoester is reacted with the 5' or 3' hydroxyl of the failure sequence between successive condensation steps in a synthesis procedure to form a 5' or 3' phosphite diester with the failure sequence. The phosphite diester substituent is inert with respect to subsequent reaction steps in the synthesis of the desired oligonucleotide product.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.