Kit for assaying activation of terminal complement cascade

US4820635A · kind A · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• Martin E. Sanders · Spring, US
• Keith A. Joiner · New Haven, US
• Michael M. Frank · Buchs, CH
• Carl H. Hammer · Gaithersburg, US

Key dates

• Filing date: Jul 31, 1987
• Grant date: Apr 11, 1989
• Expiry date: Jul 31, 2007

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/821
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A kit for assaying the activation of terminal complement cascade is disclosed. The kit includes a plurality of containers which contain a first antibody having a specificity for poly C9 neoantigen. The containers further have a second antibody which is different from the first antibody and has a specificity for a constituent of terminal complement cascade. A third antibody is optionally present which recognizes the second antibody. The kit also includes a substrate splitting enzyme, a substrate for the enzyme which produces a color reaction when split, and a SCb-9 standard microtiter plate. Pipettes and instructions for performing the assay are also included.