Transposable linkers to transfer genetic information
US4830965A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Jun 10, 1987 |
| Grant date | May 16, 1989 |
| Priority date | — |
| Expiry date | Jun 10, 2007 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/70
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Transposable linkers are DNA sequences recognized and employed by transposition proteins, including transposases, in the insertion of genetic information into the genetic architecture of an organism and in the movement of genetic information from one location to another in the genetic architecture of an organism. Such linkers, comprising the extreme ends of transposons, often referred to as left and right attachment sites, have been isolated from the intervening material in transposons to produce a basic building block comprising simply the extreme ends of the transposons fused together. Using restriction endonuclease recognition sites outside and between the left and right attachment sites it is possible to introduce desired genes directly or via cloning vehicles, into the genetic material of an organism. A transposable linker may also be used to introduce a desired gene, that has been placed in a cloning vehicle but is not, of itself, part of a normally transposable sequence, into the genetic material of an organism.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.