Solution phase nucleic acid sandwich assay
US4868105A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 11, 1985 |
| Grant date | Sep 19, 1989 |
| Priority date | — |
| Expiry date | Dec 11, 2005 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Methods and compositions are provided for rapid detection of nucleic acid sequences. The method employs two reagent sets. The first set is a labelling set comprising: (1) a first nucleic acid sequence probe having an analyte complementary region and a first recognition sequence region and (2) a labelled sequence complementary to the first recognition sequence region. The second set is a capturing set comprising: (1) a second nucleic acid sequence probe having an analyte complementary region and a second recognition sequence region, (2) a specific binding pair member conjugated to a sequence complementary to the second recognition sequence, and (3) a separating means to which is bound a complementary specific binding pair member. The sample and probes are combined under annealing conditions, followed by addition of the other reagents, separation of the bound label from the supernatant and detection of the label in either phase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.