Gram-positive expression control sequences

US4868111A · kind A · utility

Assignee

• Hoffmann-La Roche Inc. · US

Inventors

• Hermann Bujard · Heidelberg, DE
• Stuart Le Grice · Basel, CH

Key dates

• Filing date: Jun 17, 1986
• Grant date: Sep 19, 1989
• Expiry date: Jun 17, 2006

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/849
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

New gram-positive expression control DNA sequences useful in the expression of pro- or eukaryotic proteins in gram-positive organisms are provided having in the downstream direction of transcription a transcription initiation DNA sequence of gram-negative origin combined with a ribosome binding site-encoding DNA sequence, optionally a foreign gene and a transcription termination sequence. Also described are expression vectors containing these expression control DNA sequences and processes using same for the manufacture of pro- and eukaryotic polypeptides. In addition processes for the manufacture of such gram-positive expression control DNA sequences and such expression vectors are described.