Purified thermostable enzyme
US4889818A · kind A · utility
591Cited by
3References
3Claims
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Assignee
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Key dates
| Filing date | Jun 17, 1987 |
| Grant date | Dec 26, 1989 |
| Priority date | — |
| Expiry date | Jun 17, 2007 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1276
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-90,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates. The enzyme is preferably stored in a buffer of non-ionic detergents that lends stability to the enzyme.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.