Method for making nucleic acid probes
US4910300A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 5, 1988 |
| Grant date | Mar 20, 1990 |
| Priority date | — |
| Expiry date | May 5, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/55
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Modified nucleotides are provided which have the structure ##STR1## wherein R.sup.1 is a reactive group derivatizabale with a detectable label, R.sup.2 is an optional linking moiety including an amide, thioether or disulfide linkage or a combination thereof. R.sup.3 is hydrogen, methyl, bromine, fluorine or iodine, R.sup.4 is hydrogen, an acid-sensitive, base-stable blocking group or an acyl capping group, R.sup.5 is hydrogen or a phosphorus derivative, R.sup.6 is H, OH, or OR where R is a protecting group and x is an integer in the range of 1 and 8 inclusive. Methods of synthesizing the derivatizable nucleotide are disclosed, as are labeled polynucleotide probes prepared therefrom.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.