Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4965188A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 17, 1987 |
| Grant date | Oct 23, 1990 |
| Priority date | — |
| Expiry date | Jun 17, 2007 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers and extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence. The amplified sequence can be readily detected. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.