Preparation of functional human factor VIII in mammalian cells using methotrexate based selection
US4965199A · kind A · utility
265Cited by
14References
13Claims
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Key dates
| Filing date | Aug 7, 1987 |
| Grant date | Oct 23, 1990 |
| Priority date | — |
| Expiry date | Aug 7, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/10
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for producing factor VIII in recombinant mammalian host cells utilizing an expression vector containing a selectable marker DNA and an amplifiable marker DNA. The initial selection is based upon the selectable marker and subsequent amplification of factor VIII DNA and amplifiable marker DNA is conducted in cells not deficient in the amplifiable marker.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.