Non-radioactive labelled single-stranded probe, method for manufacturing it and method for detecting a specified nucleotide sequence using this probe
US4978749A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Jul 11, 1989 |
| Grant date | Dec 18, 1990 |
| Priority date | — |
| Expiry date | Jul 11, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6813
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a new probe for detecting a specified nucleic acid sequence. It consists of a recombinant DNA containing an insert complementary to the nucleic acid sequence to be detected and nuclecic acid sequences derived from the DNA (+) of phage M13, the bases which participate in the composition of this recombinant DNA, in particular the thymine groups, being replaced by 5-bromouracil groups. The invention also relates to a method for manufacturing this probe, this method comprising the culturing of cell hosts, more especially E. coli, which are auxotrophic for thymine in a medium containing a limiting concentration of thymine and containing 5-bromouracil. After the requisite incubation time, the supernatant is removed and the probe is recovered from the bacterial cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.