DNA amplification technique

US4994370A · kind A · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• Jonathan E. Silver · Bethesda, US
• Vijaya R. Keerikatte · Bethesda, US

Key dates

• Filing date: Jan 3, 1989
• Grant date: Feb 19, 1991
• Expiry date: Jan 3, 2009

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/143333
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A modification of the polymerase chain reaction (PCR) technique is described. The method allows the amplification of regions of DNA flanking a single region of known sequence, in contrast to standard PCR which requires two regions of known sequence at opposite ends of the fragment to be amplified. Various advantages of the new method are described.