Extrusion technique for producing unilamellar vesicles
US5008050A · kind A · utility
239Cited by
6References
29Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 13, 1989 |
| Grant date | Apr 16, 1991 |
| Priority date | — |
| Expiry date | Feb 13, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T428/2984
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A method for reducing the lamellarity of a population of liposomes is provided which comprises repeatedly passing the liposomes under pressure through a filter which has a pore size equal to or less than about 100 nm. In certain embodiments, the method is used to convert a population of previously formed multilamellar liposomes into a population of substantially unilamellar liposomes. In accordance with other aspects of the disclosure, liposomes are prepared directly from a lipid powder or pellet and buffer without the use of any solvents, detergents or other extraneous materials.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.