Patent · US Expired

DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides

US5010182A · kind A · utility

96Cited by

0References

7Claims

0Family size

Assignees

Chiron Corporation · US
Gist-Brocades · NL

Inventors

Anthony J. Brake · Berkeley, US
Johan A. Van Den Berg · Gouda, NL

Key dates

Filing date	Jul 28, 1987
Grant date	Apr 23, 1991
Priority date	—
Expiry date	Jul 28, 2007

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2319/02
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

DNA constructs that are useful for providing secretory expression of heterologous polypeptides in yeast comprising a DNA sequence that includes a Kluyveromyces .alpha.-factor leader sequence linked to the heterologous polypeptide by a yeast processing signal. Constructs employing the K. lactis .alpha.-factor leader and processing signal sequences, with and without spacer, linked to prochymosin are exemplified.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.