RNA ribozyme polymerases, and methods
US5037746A · kind A · utility
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Key dates
| Filing date | Mar 16, 1989 |
| Grant date | Aug 6, 1991 |
| Priority date | — |
| Expiry date | Mar 16, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/1241
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A catalytic RNA (ribozyme) derived from an intervening sequence (IVS) RNA of Tetrahymena thermophila will catalyze an RNA polymerization reaction in which pentacytidylic acid (C.sub.5) is extended by the successive addition of mononucleotides derived from a guanylyl-(3',5')-nucleotide (GpN). Cytidines or uridines are added to C.sub.5 to generate chain lengths of 10 to 11 nucleotides; longer products are also generated but at reduced efficiency. The reaction is analogous to that catalyzed by a replicase with C.sub.5 acting as the primer, GpNs as the nucleoside triphosphates, and a sequence in the ribozyme providing a template.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.