Factor VIII:C cDNA cloning and expression
US5045455A · kind A · utility
22Cited by
5References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 14, 1990 |
| Grant date | Sep 3, 1991 |
| Priority date | — |
| Expiry date | Jun 14, 2010 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Methods and compositions are provided for recombinant DNA production of Factor VIIIC and truncated derivatives thereof. Based on amino acid sequences, probes are developed for isolating messenger RNA, cDNA and/or chromosomal DNA encoding for Factor VIIIC. The Factor VIIIC gene in its entirety, a fragment thereof, or a cDNA is then used for expression of Factor VIIIC in a host. The bacteriophage .lambda.FVIII23D containing the 14.43kb EcoRI fragment was deposited at the A.T.C.C. on Jan. 4, 1984 and given Accession No. 40094. Also, the vector pSVF8-200 was deposited at the A.T.C.C. on July 17, 1985 and given Accession No. 40190.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.