Process for producing biologically active plasminogen activator in recombinant CHO cells using suspension culture and removing detrimental components from medium
US5053334A · kind A · utility
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12References
8Claims
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Key dates
| Filing date | May 9, 1990 |
| Grant date | Oct 1, 1991 |
| Priority date | — |
| Expiry date | May 9, 2010 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of producing biologically active human tissue plasminogen activator in suspension culture is provided wherein recombinant Chinese hamster ovary cells are cultured and certain components detrimental to recovery and biological activity are removed as a cell-free filtrate by cross-flow filtration. The human tissue plasminogen activator is recovered from the culture medium.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.