Yeast endopeptidase for basic amino-acid site cleavage, preparation and use

US5077204A · kind A · utility

Assignees

• Chiron Corporation · US
• The Regents of the University of California · US

Inventors

• Anthony J. Brake · Berkeley, US
• Lindley Blair · Los Angeles, US
• David Julius · San Francisco, US
• Jeremy W. Thorner · Berkeley, US

Key dates

• Filing date: Apr 8, 1988
• Grant date: Dec 31, 1991
• Expiry date: Apr 8, 2008

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/60
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and compositions are provided for endopeptidase production, enhanced efficiencies of processing in vivo and in vitro to provide for process polypeptides, and purified enzyme for in vitro processing of polypeptides. The endopeptidase is specific for dibasic amino acid sites, cleaving at the C-side of the dipeptide. The S. cerevisiae strain pYBCA-5 (truncated KEX2) was deposited at the A.T.C.C. on June 21, 1984 and given Accession No. 20717.