Labeling by simultaneous ligation and restriction
US5093245A · kind A · utility
68Cited by
1References
12Claims
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Key dates
| Filing date | Jan 26, 1988 |
| Grant date | Mar 3, 1992 |
| Priority date | — |
| Expiry date | Jan 26, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Termini of restricted double-stranded DNA fragments are modified by ligating the fragments with terminal phosphate-free double-stranded oligonucleotides having a complementary terminus in the presence of a restriction enzyme and a ligase, where joining of the complementary ends results in loss of the restriction enzyme recognition sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.