Polynucleotide determination with selectable cleavage sites
US5118605A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Sep 29, 1988 |
| Grant date | Jun 2, 1992 |
| Priority date | — |
| Expiry date | Sep 29, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Novel methods for assaying a nucleic acid analyte are provided, which employ polynucleotides having oligonucleotide sequences substantially homologous to a sequence of interest in the analyte, where the presence or absence of hybridization at a predetermined stringency provides for the release of a label from a support. Particularly, various techniques are employed for binding a label to a support, whereupon cleavage of either a single or double strand, a label may be released from a support, where the release of the label can be detected as indicative of the presence of a particular oligonucleotide sequence in a sample. The method finds use in diagnosis of disease, genetic monitoring, and analysis of nucleic acid mixtures.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.