Patent · US Expired

Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins

US5122469A · kind A · utility

472Cited by
14References
5Claims
0Family size

Assignee

Inventors

Key dates

Filing dateOct 3, 1990
Grant dateJun 16, 1992
Priority date
Expiry dateOct 3, 2010

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2500/40
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A method for culturing mammalian cells to improve production of recombinant proteins is provided. The culture media contain high levels of selected amino acids and may be supplemented with various growth factors and trace elements. In addition, the media are optionally serum-free. The media are preferably used for suspension culturing of Chinese hamster ovary cells at high densities.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.