Chemiluminescent double-triggered 1, 2-dioxetanes
US5132204A · kind A · utility
29Cited by
3References
2Claims
0Family size
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Key dates
| Filing date | May 31, 1989 |
| Grant date | Jul 21, 1992 |
| Priority date | — |
| Expiry date | May 31, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/55
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A stable 1,2-dioxetane of the formula: ##STR1## wherein X' is a phenyl group with 0 to 2 Cl or I atoms. This 1,2-dioxetane is a double-trigger substrate. The 2-methyl-4-hydroxy-naphthyl group is first removed by horseradish peroxidase. The exposed phosphate group is then removed with alkaline phosphatase. The exposed phenoxy 1,2-dioxetane decomposes to generate chemiluminescence output in immunoassays or nucleic acid hybridization assays.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.