Purification of chimeric proteins containing an IL-2 moiety by receptor-affinity chromatography
US5149788A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 24, 1988 |
| Grant date | Sep 22, 1992 |
| Priority date | — |
| Expiry date | Oct 24, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/814
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for the purification of interleukin-2 (IL-2) and chimeric proteins containing an IL-2 moiety from a wide variety of sources, including synthetic mixtures, cell culture conditioned medium and mammalian and bacterial recombinant expression systems. The methods of the invention employ IL-2 receptor-affinity adsorbents in which soluble IL-2 receptors have been immobilized on solid supports. Through the use of these affinity adsorbents, highly purified IL-2 and chimeric proteins containing an IL-2 moiety can be produced in a single step from bacterial extracts or conditioned medium. The proteins thus purified are largely free of aggregated forms, which are often present when other purification methods are used.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.