Method of synethesizing sulfurized oligonucleotide analogs
US5151510A · kind A · utility
219Cited by
1References
17Claims
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Key dates
| Filing date | Apr 20, 1990 |
| Grant date | Sep 29, 1992 |
| Priority date | — |
| Expiry date | Apr 20, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H21/00
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A method for synthesizing sulfurized oligonucleotide analogs, such as phosphorothioate and phosphorodithioate analogs, is provided that employs a thiophosphorus compound, such as a thiophosphoric, dithiophosphoric, thiophosphinic, or dithiophosphinic acid disulfide or polysulfide, as a sulfurizing agent. The method of the invention may be used to sulfurize any phosphorous(III)-containing intermediate. Preferably, the method is practiced on a commercial DNA synthesizer using phosphoramidite and/or phosphorthioamidite intermediates.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.