Purification of interleukin-2 by receptor-affinity chromatography
US5162503A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 19, 1987 |
| Grant date | Nov 10, 1992 |
| Priority date | — |
| Expiry date | May 19, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/814
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for the purification of interleukin-2 (IL-2) from a wide variety of sources, including synthetic mixtures, culture medium conditioned by natural IL-2 producing cells, and mammalian and bacterial recombinant IL-2 expression systems. The methods of the invention employ IL-2 receptor-affinity adsorbents in which soluble IL-2 receptors have been immobilized on solid supports. Through the use of these affinity adsorbents, highly purified IL-2 can be produced in a single step from bacterial extracts or conditioned medium. The IL-2 thus purified is largely free of aggregated forms, which are often present when other purification methods are used.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.