Cleavage method for acyl thiohydantoins and use of the method in C-terminal peptide sequencing
US5185266A · kind A · utility
9Cited by
4References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 15, 1991 |
| Grant date | Feb 9, 1993 |
| Priority date | — |
| Expiry date | Oct 15, 2011 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6821
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed is a method for enhancing the cleavage of an acyl thiohydantoin bond, for example, for use in C-terminal peptide sequencing. An acyl thiohydantoin is alkylated to form an adduct on the thiohydantoin, and the adduct-containing thiohydantoin is cleaved at its acyl bond by reaction with a cleaving agent under substantially anhydrous, acidic conditions. In C-terminal amino acid sequencing, the cleaved product is analyzed to identify the C-terminal amino acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.