Patent · US Expired

DNA constructs containing a kluyveromyces .alpha. factor leader sequence for directing secretion of heterologous polypeptides

US5217891A · kind A · utility

14Cited by

0References

24Claims

0Family size

Assignees

Chiron Corporation · US
Gist-Brocades · NL

Inventors

Anthony J. Brake · Berkeley, US
Johan A. Van Den Berg · Gouda, NL

Key dates

Filing date	Apr 9, 1990
Grant date	Jun 8, 1993
Priority date	—
Expiry date	Apr 9, 2010

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2319/02
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

DNA constructs that are useful for providing secretory expression of heterologous polypeptides in yeast comprising a DNA sequence that includes a Kluyveromyces .alpha.-factor leader sequence linked to the heterologous polypeptide by a yeast processing signal. Constructs employing the K. lactis .alpha.-factor leader and processing signal sequences, with and without spacer, linked to prochymosin are exemplified.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.