Rhodamine phosphoramidite compounds
US5231191A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 22, 1990 |
| Grant date | Jul 27, 1993 |
| Priority date | — |
| Expiry date | Oct 22, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07F9/24
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The invention provides a novel cleavage reagent for hydrolysing base-labile linking groups between a solid phase support and oligonucleotides. The cleavage reagent comprises a lower alkyl alcohol, water, and a non-nuccleophilic hindered alkylamine containing from 3 to 6 carbon atoms in a ratio of about 1:1:1 to about 1:3:1, respectively. An important property of the cleavage reagent is that it preserves the fluorescent characteristics of rhodamine dyes during cleavage, thereby making it possible to completely synthesize rhodamine-labeled oligonucleotides by solid phase methods. Rhodamine phosphoramidites are provided to further enhance the efficiency of synthesizing rhodamine-labeled oligonucleotides by such methods.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.