Exonuclease-resistant oligonucleotides
US5256775A · kind A · utility
435Cited by
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20Claims
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Assignee
Inventor
Key dates
| Filing date | Jun 5, 1990 |
| Grant date | Oct 26, 1993 |
| Priority date | — |
| Expiry date | Jun 5, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H21/00
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A method is provided for making 3' and/or 5' end-capped oligonucleotides so as to render the oligonucleotide resistant to degradation by exonucleases. The exonuclease degradation resistance is provided by incorporating two or more phosphoramidate and phosphorocmonothioate and/or phosphorodithioate linkages at the 5' and/or 3' ends of the oligonucleotide, wherein the number of phosphoramidate linkages is less than a number which would interfere with hybridization to a complementary oligonucleotide strand and/or which would interfere with RNAseH activity when the oligonucleotide is hybridized to RNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.