Polyacrylamide gels with improved detection of protein

US5283196A · kind A · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• Denis Francois Hochstrasser · Geneva, CH
• Carl R. Merrill · Rockville, US
• Abraham Patchornik · Nes Ziona, IL

Key dates

• Filing date: Nov 7, 1991
• Grant date: Feb 1, 1994
• Expiry date: Nov 7, 2011

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/255
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

Amine-acryloyl and -methacryloyl derivatives and 1,6-heptadiene-4-ol can be used to provide crosslinked polyacrylamide gels which are useful in protein and nucleic acid separation and detection. The gels crosslinked with the compounds of the present invention exhibit much less background silver staining than previously known acrylamide gels.