Expression of influenza a M2 protein in baculovirus
US5290686A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 31, 1991 |
| Grant date | Mar 1, 1994 |
| Priority date | — |
| Expiry date | Jul 31, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2760/16222
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to baculovirus-expressed influenza antigens, in particular, to the influenza A membrane protein, M2, expressed from Autographa Californica nuclear polyhedrosis virus (AcNPV). The present invention further relates to a method to increase the yield of baculovirus-expressed M2 proteins in host cells by culturing the recombinant baculovirus infected host cells with an amantadine-like drug. Other aspect of the present invention relate to the use of baculovirus-expressed M2 proteins in reproducible and routine assays for the seradiagnosis of influenza A virus infections as an alternative to the more burdensome complement fixation and hemagglutination tests.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.