Real-time monitoring of oxidative products from in vitro cell-biomaterial interaction using chemiluminescence

US5294541A · kind A · utility

Assignee

• The United States of America as Represented by the Secretary of the Department of Health and Human Services · US

Inventors

• David S. Kaplan · Fairfax, US
• Grace L. Picciolo · Fort Washington, US
• Edward P. Mueller · Annapolis, US

Key dates

• Filing date: Jul 13, 1992
• Grant date: Mar 15, 1994
• Expiry date: Jul 13, 2012

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/5014
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A chemiluminescence method for continuously monitoring in real time the generation of oxidative products such as hydrogen peroxide and superoxide from in vitro cell-biomaterial interactions using cell lines such as Human Leukemic cells (HL-60); tumor cell line hybridomas; cells lacking the respiratory burst such as Chronic Granulomatous Disease cells as controls; Monocytic cell lines; Primary Human cells such as monocytes, pmns, fibroblasts, endothelial cells; and whole and isolated blood cells. The oxidative products have the potential for the degradation of the biomaterial and thus this information can be used to aid in predicting the functional lifetime of the biomaterial when it is used to fabricate an implanted medical device. Further, this method may be used to determine the amount of activation of biological cells which is inferred from the amount of oxidative products. This activation level aids in the determination of the degree of the inflammatory response and thus influences the degree of acceptance of the biomaterial. Thus, toxicity and biocompatability tests could be supported by these measurements.