Methods for nucleic acid amplification
US5314809A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 10, 1993 |
| Grant date | May 24, 1994 |
| Priority date | — |
| Expiry date | Mar 10, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/706
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for enhanced specificity and sensitivity of nucleic acid amplification. The methods are simplified nested amplification procedures wherein both inner and outer primer pairs are present in the amplification reaction mixture. According to the methods, the thermocycling profile, as well as the sequences, length, and concentration of amplification primers, is modified to regulate which primers are annealed and extended on the target during any particular amplification cycle. The methods described are particularly suitable in PCR amplifications and have numerous applications in molecular biology, medical diagnostics, and forensics.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.