Method and apparatus for measuring binding affinity
US5324633A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 22, 1991 |
| Grant date | Jun 28, 1994 |
| Priority date | — |
| Expiry date | Nov 22, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/823
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and device for measuring the binding affinity of a receptor to a ligand. According to one aspect of the invention, arrays of polymers are synthesized or immobilized on a substrate (212). The array of polymers is exposed to a fluorescently-labelled receptor in solutions of varying concentration. The fluorescence intensity of the labelled receptor is measured by way of, e.g., a photon counter using a confocal microscope (316). Binding affinity is determined through analysis of the relationship between fluorescence intensity and the solution concentration of the receptor. On-rates are measured as a kinetic increase in surface fluorescence intensity, and the on-rate constant rate extracted from fits to the data.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.