Method of preparing fusion proteins

US5358857A · kind A · utility

Assignees

• The General Hospital Corporation · US
• Hoechst GmbH · DE

Inventors

• Siegfried Stengelin · Frankfurt am Main, DE
• Paul Habermann · Solms, DE
• Eugen Uhlmann · Königstein, DE
• Brian Seed · Boston, US

Key dates

• Filing date: Jun 9, 1993
• Grant date: Oct 25, 1994
• Expiry date: Jun 9, 2013

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/75
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Fusion proteins are obtained in high yields if a mixed oligonucleotide is constructed which codes for the ballast constituent of the fusion protein. The oligonucleotide mixture is introduced in a vector in such a manner that it is functionally linked to a regulatory region and to the structural gene for the desired protein. Appropriate host cells are transformed with the plasmid population obtained in this manner and the clones producing a high yield of coded fusion protein are selected.