Method of isolating lung cell line
US5364785A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 11, 1993 |
| Grant date | Nov 15, 1994 |
| Priority date | — |
| Expiry date | May 11, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2503/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A novel bronchial or bronchiolar epithelial cell from normal neonatal rat lung has been isolated, established and maintained for multiple passages in the absence of serum, without undergoing crisis or senescence. By careful manipulation of the nutritional/hormonal microenvironment we have been able to select, from a heterogeneous population, a single epithelial cell type which can maintain highly differentiated features in vitro. This cell type has characteristics of bronchiolar epithelial cells. A clonal line, RL-65, has been selected and observed for more than 2 years in continuous culture. It has been characterized by ultrastructural, morphological and biochemical criteria.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.