Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5366860A · kind A · utility
215Cited by
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10Claims
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Key dates
| Filing date | Sep 29, 1989 |
| Grant date | Nov 22, 1994 |
| Priority date | — |
| Expiry date | Sep 29, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC09B11/24
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A spectrally resolvable set of rhodamine dyes are provided for use in the chain termination method of nucleic acid sequencing. A different rhodamine dye from the group consisting of tetramethylrhodamine, rhodamine X, rhodamine 6G, and rhodamine 110 is attached to the base of each of the dideoxynucleotides used in the sequencing method by way of an alkynylamino linker. Preferably, the labeled dideoxynucleotides are incorporated into the growing DNA chains by Taq DNA polymerase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.