Oligonucleotides with selectably cleavable and/or abasic sites
US5367066A · kind A · utility
516Cited by
4References
23Claims
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Assignee
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Key dates
| Filing date | Jul 24, 1991 |
| Grant date | Nov 22, 1994 |
| Priority date | — |
| Expiry date | Jul 24, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/683
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A modified polynucleotide containing at least one cleavable or abasic site as shown below. ##STR1## DNA.sub.1 is a first segment of DNA; DNA.sub.2 is a second segment of DNA; and R.sub.m is C.sub.1 to C.sub.16 alkylene or an oxytheylene oligomer --(CH.sub.2 CH.sub.2 O).sub.z -- where z is an interger in the range of 1 to 16 inclusive, and R.sub.n is selected from the group consisting of ##STR2## Such polynucleotides are useful in solid phase hybridizations because they permit the release of a label from the solid support after the hybridization reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.