High resolution DNA sequencing method using low viscosity medium
US5374527A · kind A · utility
82Cited by
2References
17Claims
0Family size
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Inventor
Key dates
| Filing date | Jan 21, 1993 |
| Grant date | Dec 20, 1994 |
| Priority date | — |
| Expiry date | Jan 21, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A DNA sequencing method for use in sequencing a DNA target sequence up to 300 bases, preferably up to 500 bases or greater in length, by electrophoretically separating a mixture of single-stranded DNA sequencing fragments in a capillary tube. The method employs an aqueous denaturing solution comprising between about 4 and about 7 weight percent linear polyacrylamide molecules having an average molecular weight of between about 20 and about 100 kDa. The low-viscosity of the solution allows rapid loading and reloading of such solution into the capillary tube.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.